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Lea Pogacnik. Completed the Doktor (PhD) in School of Environmental Sciences in 2001 by Univerza v Novi Gorici and Bachelor in University Study Programme of Chemistry in 1995 by Univerza v Ljubljani. Is Associate Professor in Univerza v Ljubljani Biotehniska fakulteta. Published 21 articles in journals. Has received 4 awards and/or honors. She has menthored 2 PhD students, 13 MSc students and 36 BSc students. Her reaserch fields are: -simultaneous isolation and biochemical characterization of chatepsin B, H and S from pork spleen -research work on the field of application of thermal lens spectrometry in analytical chemistry -development and optimization of photothermal biosensor and its application for detection of low concentrations of organophosphate and carbamate pesticides in water, fruit juices and vegetables - determination of sample toxicity -determination of correlation between detected toxicity of samples by a biosensor with LD50 values, determined on sample animals -taking part in soil sample analysis, taken in the neighbourhood of planned highways -measurement of dust and heavy metals in emissions from Cinkarna Celje -introduction of improved system of practical classes (contemporary study, students` self-evaluation, etc.) -taking part at knowledge evaluation of the first year students at various faculty programs (Biotechnical Faculty, Medical Faculty, Faculty for Chemistry and Chemical Engineering) -optimization of growth conditions of thermophilic microorganisms Archaea Aeropyrum pernix and study of their shape under electronic microscope -optimization of chemoluminescence assay for determination of antioxidative capacity in food extracts -determination of antioxidative potential, total phenols, vitamin C content and qualitative identification of several phenolic acids and flavonoids in the fresh and dried medicinal herbs and invasive knotweed species by HPLC-DAD and HPLC-MS system -studying the influence of environmental factors on content of total phenols, vitamin C and antioxidative activity of rose hip berries - studying the influence of resevratrol on oxidative stability of oil - characterising bioactivities from invasive knotweed species - studying the neuroprotective properties of polyphenols
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Identification

Personal identification

Full name
Lea Pogacnik

Citation names

  • Pogacnik, Lea

Author identifiers

Ciência ID
EA1F-7161-40DB
ORCID iD
0000-0003-1008-0633
Scopus Author Id
6602496819

Knowledge fields

  • Exact Sciences - Chemical Sciences - Analytical Chemistry
  • Engineering and Technology - Other Engineering and Technologies - Food and Beverages
  • Medical and Health Sciences - Health Sciences - Nutrition, Dietetics

Languages

Language Speaking Reading Writing Listening Peer-review
Slovenian Advanced (C1) Advanced (C1) Advanced (C1) Advanced (C1)
English Advanced (C1) Advanced (C1) Advanced (C1) Advanced (C1)
German Intermediate (B1) Intermediate (B1) Intermediate (B1) Intermediate (B1)
Portuguese Beginner (A1) Beginner (A1) Beginner (A1) Beginner (A1)
Education
Degree Classification
2001
Concluded
 School of Environmental Sciences (Doktor (PhD))
Univerza v Novi Gorici, Slovenia
"Development and Application of Photothermal Biosensor for Detection of Organophosphate and Carbamate Pesticides" (THESIS/DISSERTATION)
 N/A
1995
Concluded
 University Study Programme of Chemistry (Bachelor)
Univerza v Ljubljani, Slovenia
"Simultaneous isolation of chatepsin B, H and S from pork spleen" (THESIS/DISSERTATION)
 N/A
Affiliation

Science

Category
Host institution 		Employer
1999/05/01 - 2000/11/30 Researcher (Research) Univerza v Novi Gorici, Slovenia
1995/04/15 - 1999/04/30 Researcher (Research) Institut Jožef Stefan, Slovenia

Teaching in Higher Education

Category
Host institution 		Employer
2015/12/16 - Current Associate Professor (University Teacher) Univerza v Ljubljani Biotehniska fakulteta, Slovenia
2000/12/01 - 2015/12/15 Assistant Professor (University Teacher) Univerza v Ljubljani Biotehniska fakulteta, Slovenia
Projects

Grant

Designation Funders
2019 - 2022 Lactic acid fermentation for enrichment of microalgae biomass with new nutrients
J4-1773 (C)
Researcher
1999 - 2022 Biochemical and biophysical-chemical characterization of natural products
P4-0121
P4-0121
Researcher
N/A
2019 - 2021 Identification and caracterisation of bioactivities from natural sources
BI-RU/19-20-046
Principal investigator
2016 - 2019 Cultivation of industrial hemp (Cannabis sativa L.) in Slovenia
V4-1611 (D)
Researcher
2011/07/01 - 2014/06/30 Oligomers of amyloidogenic proteins from a to z: biophysical properties, structure, function and mutual interactions (basic research project)
J7-4050 (D)
J7-4050 (D)
Integration into Research Grant Fellow
N/A
2012 - 2013 Telemonitoring and Telediagnostic for Life Sciences (ERASMUS IP)
12-EIP-RO BRASOV01-BIS
12-EIP-RO BRASOV01-BIS
Principal investigator
N/A
2009 - 2010 Bioanalytical methods for life sciences (ERASMUS IP)
51388-IC-1-2007-1-RO-ERASMUS-EUCX-1
51388-IC-1-2007-1-RO-ERASMUS-EUCX-1
Principal investigator
N/A
2004 - 2007 6487Extremophiles as a source of novel bioactive substances
J1-6487
J1-6487
Researcher
N/A
1998 - 2001 Photothermal spectrometry in the samples with high light scattering
J1-0091
J1-0091
Researcher
N/A
Outputs

Publications

Book
  1. Pogacnik, Lea. Chemistry with Biochamistry for Students of Agronomy and Animal Sciences. Slovenia. 2017.
Journal article
  1. Pogacnik, Lea; Bergant, Tina; Skrt, Mihaela; Poklar Ulrih, Nataša; Viktorova, Jitka; Ruml, Tomas. "In vitro comparison of bioactivities of Japanese and Bohemian knotweed ethanol extracts". Foods (2020):
    In press
  2. Pogacnik, Lea. "BIOACTIVE SUBSTANCES FROM INVASIVE KNOTWEED SPECIES". Journal of EcoAgri Tourism 16 1 (2020): 21-25.
    In press
  3. Pogacnik, Lea; Silva FM Rui. "Poyphenols – between neuroprotection and neurotoxicity". Acta agriculturae Slovenica 115 2 (2020): 343-353.
    Open access • In press
  4. Mariana Gois Ruivo da Silva; Mihaela Skrt; Draženka Komes; Nataša Poklar Ulrih; Lea Pogacnik. "Enhanced Yield of Bioactivities from Onion (Allium cepa L.) Skin and Their Antioxidant and Anti-a-Amylase Activities". International Journal of Molecular Sciences 21 8 (2020): 2909-2909. https://doi.org/10.3390/ijms21082909.
    10.3390/ijms21082909
  5. Lea Pogacnik; Ajda Ota; Nataša Poklar Ulrih. "An Overview of Crucial Dietary Substances and Their Modes of Action for Prevention of Neurodegenerative Diseases". Cells 9 3 (2020): 576-576. https://doi.org/10.3390/cells9030576.
    10.3390/cells9030576
  6. RFM Silva; Lea Pogacnik. "Polyphenols from Food and Natural Products: Neuroprotection and Safety". Antioxidants (2020): https://www.mdpi.com/2076-3921/9/1/61.
    10.3390/antiox9010061
  7. Silva, R.F.M.; Pogacnik, L.. "Food, polyphenols and neuroprotection". Neural Regeneration Research 12 4 (2017): 582-583. http://www.scopus.com/inward/record.url?eid=2-s2.0-85019063607&partnerID=MN8TOARS.
    10.4103/1673-5374.205096
  8. Pogacnik, L.; Rogelj, A.; Ulrih, N.P.. "Chemiluminescence Method for Evaluation of Antioxidant Capacities of Different Invasive Knotweed Species". Analytical Letters 49 3 (2016): 350-363. http://www.scopus.com/inward/record.url?eid=2-s2.0-84949935120&partnerID=MN8TOARS.
    10.1080/00032719.2014.979361
  9. Pogacnik, L.; Pirc, K.; Palmela, I.; Skrt, M.; Kwang, K.S.; Brites, D.; Brito, M.A.; Ulrih, N.P.; Silva, R.F.M.. "Potential for brain accessibility and analysis of stability of selected flavonoids in relation to neuroprotection in vitro". Brain Research 1651 (2016): 17-26. http://www.scopus.com/inward/record.url?eid=2-s2.0-84990903127&partnerID=MN8TOARS.
    10.1016/j.brainres.2016.09.020
  10. Pirc, K.; Škarabot, M.; Pogacnik, L.; Žerovnik, E.; Ulrih, N.P.. "The effect of tyrosine residues on a-synuclein fibrillation". Acta Chimica Slovenica 62 1 (2015): 181-189. http://www.scopus.com/inward/record.url?eid=2-s2.0-84927667378&partnerID=MN8TOARS.
    10.17344/acsi.2014.882
  11. Pogacnik, L.; Ulrih, N.P.. "Application of optimized chemiluminescence assay for determination of the antioxidant capacity of herbal extracts". Luminescence 27 6 (2012): 505-510. http://www.scopus.com/inward/record.url?eid=2-s2.0-84871799241&partnerID=MN8TOARS.
    10.1002/bio.1384
  12. Pogacnik, L.; Cigic, B.. "How to motivate students to study before they enter the lab". Journal of Chemical Education 83 7 (2006): 1094-1098. http://www.scopus.com/inward/record.url?eid=2-s2.0-33745587755&partnerID=MN8TOARS.
  13. Pogacnik, L.; Franko, M.. "Photothermal bioanalytical methods for pesticide toxicity testing". Arhiv za Higijenu Rada i Toksikologiju 54 3 (2003): 197-205. http://www.scopus.com/inward/record.url?eid=2-s2.0-0344441718&partnerID=MN8TOARS.
  14. Mežnar, K.; Pihlar, B.; Pogacnik, L.; Franko, M.. "Optimised calibration procedure for bioanalytical determination of organophosphate pesticides in apple juices by immobilised AChE". Acta Chimica Slovenica 50 3 (2003): 383-394. http://www.scopus.com/inward/record.url?eid=2-s2.0-0141617340&partnerID=MN8TOARS.
  15. Pogacnik, L.; Franko, M.. "Detection of organophosphate and carbamate pesticides in vegetable samples by a photothermal biosensor". Biosensors and Bioelectronics 18 1 (2003): 1-9. http://www.scopus.com/inward/record.url?eid=2-s2.0-0037210445&partnerID=MN8TOARS.
    10.1016/S0956-5663(02)00056-8
  16. Franko, M.; Trebše, P.; Pogacnik, L.. "Laser-induced degradation of organophosphates and monitoring of their toxicity by cholinesterase biosensors". Critical Reviews in Analytical Chemistry 33 4 (2003): 285-290. http://www.scopus.com/inward/record.url?eid=2-s2.0-1642489080&partnerID=MN8TOARS.
    10.1080/714037678
  17. Pogacnik, L.; Franko, M.. "Validation of different commercially available cholinesterases for pesticide toxicity test". Annali di Chimica 92 1-2 (2002): 93-101. http://www.scopus.com/inward/record.url?eid=2-s2.0-0036355158&partnerID=MN8TOARS.
  18. Pogacnik, L.; Franko, M.. "Optimisation of FIA system for detection of organophosphorus and carbamate pesticides based on cholinesterase inhibition". Talanta 54 4 (2001): 631-641. http://www.scopus.com/inward/record.url?eid=2-s2.0-0141497562&partnerID=MN8TOARS.
    10.1016/S0039-9140(01)00314-9
  19. Pogacnik, L.; Franko, M.. "Determination of organophosphate and carbamate pesticides in spiked samples of tap water and fruit juices by a biosensor with photothermal detection". Biosensors and Bioelectronics 14 6 (1999): 569-578. http://www.scopus.com/inward/record.url?eid=2-s2.0-0039293206&partnerID=MN8TOARS.
    10.1016/S0956-5663(99)00029-9
Thesis / Dissertation
  1. Pogacnik, Lea. "Development and application of photothermal biosensor for detection of organophosphate and carbamate pesticides". PhD, 2001.
Activities

Interview (tv / radio show)

Program Topic
2016/09/22 - Current Slovene national TV SLO1 Let's bite the science Can the invasive plants be beneficial?

Mentoring / Tutoring

Topic Student name
2018 - 2019 Optimization of quercetin extraction from onion skin : determination of antioxidant capacity and anti-diabetic activity Mariana Goios Ruivo da Silva
2018 - 2019 Preparation and characterisation of cianobacteria species Arthrospira platensis extracts before and after the lactic acid fermentation Aleksandra Mrak
2018 - 2019 The effect of tea (Camellia sinensis L.) preparation on the flavanol content and antioxidant capacity Monika Graj
2017 - 2019 In vitro characterization of bioactivities of rhizome and flower extracts of Japanese (Fallopia japonica) and Bohemian (Fallopia x bohemica) knotweed Tina Bergant
2017 - 2018 Bioactive effect of non-native knotweed root extracts (Fallopia japonica, Fallopia sachalinensis and Fallopia x bohemica) Sara Recek
2017 - 2018 Identification and quantification of polyphenolic compounds from extracts of different knotweed species Fallopia japonica, F. sachalinensis and F. x bohemica Martina Korencan
2016 - 2018 Impact of resveratrol on oil stability Ursa Mrsnik
2012 - 2016 Stability of anthocyanins during simulated in vitro digestion of pomegranate juice (Punica granatum L.) Tina Klepac
2014 - 2015 Antioxidants and their efficiency in different tissues of invasive alien knotweed species Anze Rogelj
2011 - 2015 The molecular mechanism of [alpha]-synuclein binding to model lipid membranes Katja Pirc
2008 - 2009 Determination of the antioxidants in medicinal herbs Tadeja Jakus
2008 - 2009 Influence of environmental factors on content of total phenols, vitamin C and antioxidative activity of rose hip berries Žiga Klemenc
2008 - 2009 The impact of ripening on antioxidative activity in dog-rose berries Sandra Potocnik
2004 - 2009 Cloning of genes for the biosynthesis of tetracyclines from selected bacteria of Actinomycetales genus Urska Lesnik
2007 - 2008 Optimisation of luminiscent method for detection of antioxidative activity in plant extracts Tina Klepac
Distinctions

Award

2015 Prešeren's award for the best MSc thesis - Antioxidants and their efficiency in different tissues of invasive alien knotweed species
Univerza v Ljubljani Biotehniska fakulteta, Slovenia
2014 Poster award - The influence of experimental conditions on antioxidants during in vitro digestion of pomegranate juice
2009 Krka's aword for exceptional research achievements - Cloning of genes for the biosynthesis of tetracyclines from selected bacteria of Actinomycetales genus
1998 Frst prize for contribution "Determination of Organophosphorus and Carbamate Pesticides in Fruit Juices by a Biosensor with Photothermal detection