Bruno Manadas has a degree in biochemistry and a PhD in Cell Biology. He became a full PI at CNC-UC in 2017, leading a team with a background in medicine, psychology, biochemistry, chemistry, biotechnology, and pharmaceutical biotechnology. He has over 130 publications, 3 projects from FCT as PI, one LaCAixa project as PI, 6 as co-PI, one European Project as co-PI, is the head of the Mass Spectrometry node at CNC of the National Mass Spectrometry Network since 2006, is an invited peer-reviewer of over 20 journals, Associated Editor of Frontiers in Molecular Neuroscience, and Frontiers in Psychiatry, Member of the European Proteomics Association (EuPA) education committee since 2021, and board member of the Portuguese Proteomics Association (Procura). Bruno Manadas was also enrolled in the settlement of the central hospital biobank with his expertise in sample storage methods for large screening approaches. His academic activities also include being responsible for the curricular units of Neurobiology, Biology of Aging, Proteomics, and Molecular Diagnosis in the last 10 years at the Portuguese Catholic University and responsible for advanced courses at the CNC PhD program (one sponsored by the European ESFRI Instruct network). His major focus has been on elucidating cell biology mechanisms and translational biomarker research using mass spectrometry-based approaches. One example of cell biology research was the identification of cofilin through proteomics screenings and its validation as a central protein in the mechanomodulation mechanisms of mesenchymal stem cells, co-headed with Dr Mario Grãos (Domingues, C., ...., B. Manadas* and M. Graos* (2020). "Cofilin-1 Is a Mechanosensitive Regulator of Transcription." Front Cell Dev Biol 8(678): 678. DOI 10.3389/fcell.2020.00678). The identification of new biomarkers for Parkinsons disease using in vitro cellular secretome proteomics screening and validation of the biomarkers in plasma samples from PD patients is one example of translational biomarkers research being performed in his lab (Anjo, S. I., ...., B. Manadas (2020). "A different vision of translational research in biomarker discovery: a pilot study on circulatory mitochondrial proteins as Parkinson's disease potential biomarkers." Transl Neurodegener 9: doi: 11.10.1186/s40035-020-00188-0). Bruno Manadas has also been coordinating the development of new MS-based approaches, p.e., to study protein oxidation and its relevance in cell biology mechanisms and biomarkers in oxidative stress conditions (Anjo, S. I., ..., B. Manadas (2019). "oxSWATH: An integrative method for a comprehensive redox-centered analysis combined with a generic differential proteomics screening." Redox Biol 22: 101130. DOI 10.1016/j.redox.2019.101130), and recently identified two subgroups in a clinical homogenous population of Mild Cognitive Impairment due to Alzheimer’s disease (Moutinho, D., Mendes, V.M., Caula, A. et al. Pathophysiological subtypes of mild cognitive impairment due to Alzheimer’s disease identified by CSF proteomics. Transl Neurodegener 13, 19 (2024). https://doi.org/10.1186/s40035-024-00412-1). Bruno Manadas also has experience in student supervision with 2 concluded PhDs, 3 ongoing PhD students as supervisor, 4 ongoing PhD students as co-supervisors, and 23 concluded Master thesis. Current funding includes a LaCaixa Impulse Project as PI; CoLab(AccellBio), with a research contract and 100k€ for consumables; a Santa Casa da Misericórdia project as co-PI, an European Project as co-PI and services to support ongoing and future PhD projects.