???global.info.a_carregar???
Identificação

Identificação pessoal

Nome completo
Filipa Sofia Silva Engrola

Nomes de citação

  • Engrola, Filipa
  • Filipa S. S. Engrola

Identificadores de autor

Ciência ID
4219-13FE-6152
ORCID iD
0000-0002-7066-5126

Endereços de correio eletrónico

  • f.engrola@campus.fct.unl.pt (Profissional)

Telefones

Telefone
  • 212948300 Ext.: 10962 (Profissional)

Websites

Formação
Grau Classificação
2021/05/01 - 2025/05/01
Em curso
Doctoral Program in Biochemistry (Doutoramento)
Especialização em Structural Biochemistry
Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal

REQUIMTE Unidade de Ciências Biomoleculares Aplicadas, Portugal
2017/09 - 2019/11
Concluído
Bioquímica (Mestrado)
Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
"Bacterial arsenite oxidation at the molecular level " (TESE/DISSERTAÇÃO)
2014/09 - 2017/07
Concluído
Bioquímica (Licenciatura)
Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
"Phase transformations and controlled delivery of an ionic liquid derived from Ibuprofen" (TESE/DISSERTAÇÃO)
Percurso profissional

Ciência

Categoria Profissional
Instituição de acolhimento
Empregador
2021/05/01 - Atual Estagiário de Investigação (Investigação) Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
(...)
2020/05/01 - 2021/04/30 Estagiário de Investigação (Investigação) Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
REQUIMTE Unidade de Ciências Biomoleculares Aplicadas, Portugal
2018/09 - 2019/11 Estagiário de Investigação (Investigação) Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
REQUIMTE Unidade de Ciências Biomoleculares Aplicadas, Portugal
2017/07/01 - 2017/07/31 Estagiário de Investigação (Investigação) Universidade de Lisboa Instituto Superior Técnico, Portugal
Universidade de Lisboa Instituto Superior Técnico, Portugal
Projetos

Bolsa

Designação Financiadores
2020/05/01 - 2021/04/30 BACTERIAL ABC-TYPE MULTITASK ENERGIZERS: ONE ATPASE FITS ALL?
Bolseiro de Investigação
REQUIMTE Unidade de Ciências Biomoleculares Aplicadas, Portugal
Fundação para a Ciência e a Tecnologia
Em curso
Produções

Publicações

Capítulo de livro
  1. Engrola, Filipa; Paquete-Ferreira, João; Santos-Silva, Teresa; Correia, Márcia A. S.; Leisico, Francisco; Santos, Marino F. A.. "Screening of Buffers and Additives for Protein Stabilization by Thermal Shift Assay: A Practical Approach". In Advanced Methods in Structural Biology, 199-213. Springer US, 2023.
    Publicado • 10.1007/978-1-0716-3147-8_11
  2. Paquete-Ferreira, João; Leisico, Francisco; Correia, Márcia A. S.; Engrola, Filipa; Santos-Silva, Teresa; Santos, Marino F. A.. "Using Small-angle X-ray Scattering to Characterize Biological Systems: A General Overview and Practical Tips". In Advanced Methods in Structural Biology, 381-403. Springer US, 2023.
    10.1007/978-1-0716-3147-8_22
Documento de trabalho
  1. Engrola, Filipa; Correia, Márcia; Cameron Watson; Carlos C. Romão; Veiros, Luis; Maria Joao Romao; Santos-Silva, Teresa; Joanne M. Santini. 2023. "Arsenite oxidase in complex with antimonite and arsenite oxyanions - insights into the catalytic mechanism (under review)".
Poster em conferência
  1. Engrola, Filipa; Correia, Márcia; Viegas, Aldino; Romão, Maria; Santos-Silva, Teresa. "Unveiling the interaction of arsenite oxidase with electron parterns – a structural and biophysical approach". Trabalho apresentado em CCP4 Study Weekend 2024: Decision making in MX - how to be a productive structural biologist, 2024.
  2. Engrola, Filipa; Correia, Márcia; Cameron Watson; Joanne M. Santini; Viegas, Aldino; Maria Joao Romao; Santos-Silva, Teresa. "A biophysical and structural approach to shed light on arsenite oxidase reaction mechanism". Trabalho apresentado em 9th National Meeting of Synchrotron Radiation Users - ENURS 2023, Portugal, 2023.
  3. Engrola, Filipa; Correia, Márcia; Cameron Watson; Joanne M. Santini; Viegas, Aldino; Maria Joao Romao; Santos-Silva, Teresa. "Arsenite oxidase interaction with substrates and partners: a structural and biophysical characterization". Trabalho apresentado em 8th International School of Biological Crystallization - ISBC 2023, Spain, 2023.
  4. Engrola, Filipa; Lora Denson; Pinheiro, Benedita; Ci Chu; Santos-Silva, Teresa; Manuel Etzkorn; Viegas, Aldino. "Production of human norepinephrine transporter - hNET - in insect cells". Trabalho apresentado em Joint Instruct-iNEXT Course: Integrating Structural Biology Techniques, 2022.
  5. Engrola, Filipa; Correia, Márcia; Viegas, Aldino; Maria Joao Romao; Santos-Silva, Teresa. "Arsenite oxidase – a structural and biophysical study from the reaction intermediates to the electron acceptors". Trabalho apresentado em Meeting with UCIBIO Scientific Advisory Board, 2022.
  6. Engrola, Filipa; Correia, Márcia; Cameron Watson; Joanne M. Santini; Maria Joao Romao; Santos-Silva, Teresa. Autor correspondente: Santos-Silva, Teresa. "How to choose a good electron partner for arsenite oxidase?". Trabalho apresentado em MOTEC 2021 — 12th Molybdenum and Tungsten Enzymes Conference, 2021.
  7. Engrola, Filipa S. S.; Correia, Márcia; Joanne M. Santini; Cameron Watson; Romão, Maria; Santos-Silva, Teresa. "New insights on the catalytic mechanism of arsenite oxidase". Trabalho apresentado em In situ Structural Biology: From Cryo-EM to Integrative Modelling, 2020.
  8. Engrola, Filipa; Romão, Maria; Correia, Márcia; Joanne M. Santini; Cameron Watson; Teresa Sacadura Santos-Silva. "Arsenite oxidase: Structural and functional insigths on the electron transfer pathway". Trabalho apresentado em Instruct Biennial Structural Biology Conference: Deeper into the Cell, 2019.
Tese / Dissertação
  1. Engrola, Filipa Sofia Silva. "Bacterial arsenite oxidation at the molecular level". Mestrado, Universidade Nova de Lisboa, 2019. http://hdl.handle.net/10362/89008.

Artística / Interpretação

Gravação vídeo
  1. Engrola, Filipa S. S.; Andreia C. P. Fernandes. 2019. VOX POP - O que a ciência tem para nos dar. Rock the Elements - The Challange.
  2. Engrola, Filipa S. S.; Luís Miguel Mariano Domingues Filipe. 2019. RockTheElements - The Dark Side of The Periodic Table. Rock the Elements - The Challange.
  3. Engrola, Filipa S. S.; Luís Miguel Mariano Domingues Filipe; Andreia Lamy. 2016. Prémios Nobel- VOX POP.
Atividades

Apresentação oral de trabalho

Título da apresentação Nome do evento
Anfitrião (Local do evento)
2023/06/07 Enzyme-substrate & protein-protein interaction: the case study of Arsenite oxidase (Flash presentation) Affinity 2023 - 25th meeting of the International Society for Molecular Recognition, Portugal
(Lisboa, Portugal)
2023/05/26 Arsenite oxidase interaction with substrates and partners: a structural and biophysical characterization (Flash Presentation) 8th International School of Biological Crystallization - ISBC 2023, Spain
(Granada, Espanha)
2022/07/16 Arsenite oxidase: a complex enzyme to tackle a complex environmental problem: arsenite water contamination 2nd National Crystallographic Meeting
Técnico Lisboa - Universidade de Lisboa (Lisboa, Portugal)
2021/09/21 Flash talk - How to choose a good electron partner for arsenite oxidase? 12th Molybdenum and Tungsten Enzymes Conference, MoTEC21
Virtual (Marseille, França)
2019/07 Arsenite oxidase: from the substrate analogue to the electron acceptors – Structural and functional insights The 8th Meeting of Portuguese Synchrotron Radiation Users (ENURS 2019)
Faculty of Sciences of University of Porto (FCUP) (Porto, Portugal)
2019/03 Bacterial arsenite oxidation at the molecular level – Protein crystallography and Small Angle X-ray Scattering VII Encontro Nacional de Estudantes de Química 2019 / "VII National Meeting of Chemistry Students" (ENEQUI '19)
NOVA School of Science and Technology (FCT NOVA) (Caparica, Portugal)
2019/02/15 Bacterial arsenite oxidation at the molecular level – Protein crystallography and Small Angle X-ray Scattering Jornadas Intercalares das Dissertações Anuais dos Mestrados do Departamento de Química e do Departamento de Ciências da Vida (JIM)
Universidade Nova de Lisboa (Portugal)
2018/11/28 Amor à Primeira Vista - as hormonas / Love at first sight - the hormones World's Biggest Chemistry Class Cascais 2018/ A Maior Aula de Química do Mundo Cascais 2018
CASCAIS 2018 - CAPITAL EUROPEIA DA JUVENTUDE (Cascais, Portugal)

Orientação

Título / Tema
Papel desempenhado
Curso (Tipo)
Instituição / Organização
2023/11/01 - 2024/01/01 Structural studies in an arsenite oxidase - Rotações laboratoriais em Técnicas Biomoleculares I
Coorientador de Guilherme Oliveira
Biochemistry (Curso de aperfeiçoamento / especialização)
Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2022/07 - 2022/07 ESCOLA DE VERÃO UCIBIO 2022 Iniciação à Investigação em Ciências Biomoleculares Aplicadas - Estudos estruturais e funcionais com duas enzimas de molibdénio
Coorientador
Biochemistry (Iniciação científica)
Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2022/02 - 2022/06 Projecto final de Bioquímica - Compreender o mecanismo enzimático para a detoxificação de arsénio com vista à produção de novos biossensores
Coorientador
Bioquímica (Outra)
Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal

Organização de evento

Nome do evento
Tipo de evento (Tipo de participação)
Instituição / Organização
2019/04 - Atual Volunteer in hosting and organizing an event at EXPO-FCT 2019. This annual event is destined to present the faculty and its educational offer, which aims to facilitate the choice of higher education by young pre-university students, through demonstrations and activities in science and engineer areas. (2019/04/23)
Exposição (Coorganizador)
Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2017/09/01 - Atual Founding member of the “Núcleo de estudantes de Bioquímica FCT-UNL (NuBioq)” (2017/09/01)
Exposição (Membro da Comissão Organizadora)
Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2016/11/25 - Atual This lecture aimed to clarify how it is to work within the scientific community, tips on how to build a solid career and how to deal with the struggles. Pedro Viana Baptista, Professor at FCT-NOVA and Helena Santos, Professor at ITQB were the invited guests. (2016/11/25)
Seminário (Presidente da Comissão Organizadora)
Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2019/03 - 2019/03 Laboratory experiments planning for primary school students. (2019/03 - 2019/03)
Exposição (Membro da Comissão Organizadora)
Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal

Agrupamento de Escolas Professor Armando de Lucena, Portugal
2016/06 - 2017/06 Former Director "área educativa do núcleo de estudantes de Bioquímica FCT-NOVA". Charity, science communication or mentoring are some of the activities developed to help the student body. (2016/06)
Exposição (Presidente da Comissão Organizadora)

Participação em evento

Descrição da atividade
Tipo de evento
Nome do evento
Instituição / Organização
2022/07/08 - Atual The FEBS Congress is one of the most visible activities of FEBS. As one of the largest bio-congresses in Europe, featuring up to approx. 2500 attendees, the Congress provides a platform for international scientific exchange and showcases the newest developments in biochemistry, molecular biology and related areas. The Congress comprises plenary lectures presented by outstanding scientists including Nobel Laureates, and a range of subject-specific symposia to provide the latest updates in different areas of bioscience research.
Conferência
IUBMB-FEBS-PABMB 2022 CONGRESS
Federation of European Biochemical Societies, Reino Unido
2019/07/28 - Atual Researchers, users of synchrotron radiaton from any infrastructure and research field present their recent works both by oral and poster communications.
Conferência
The 8TH Meeting of Portuguese Synchrotron Radiation Users (ENURS 2019)
Universidade do Porto Faculdade de Ciências, Portugal
2018/11/28 - Atual Key speaker at the World Guinness Record "World's Biggest Chemistry Class Cascais 2018/A Maior Aula de Química do Mundo Cascais 2018". A science communication event that took place in Cascais, where 1440 elementary school students learned about chemistry.
Seminário
World's Biggest Chemistry Class Cascais 2018/ A Maior Aula de Química do Mundo Cascais 2018
2024/01/03 - 2024/01/05 CCP4 Study Weekend 2024 - “Decision making in MX – how to be a productive structural biologist”
Conferência
CCP4 Study Weekend 2024 - “Decision making in MX – how to be a productive structural biologist”
University of Nottingham School of Biosciences, Reino Unido
2023/06/21 - 2023/06/21 The meeting provides an opportunity to hear about the developments upgrades and new opportunities at the European Synchrotron Research Facility in Grenoble France and the Alba Synchrotron Light Source in Barcelona, Spain. The meeting also provides opportunities for users to share their recent research via oral or poster presentations.
Encontro
9th National Meeting of Synchrotron Radiation Users - ENURS 2023, Portugal
Instituto Politécnico de Leiria, Portugal
2023/06/05 - 2023/06/07 Global scientific event that covers fundamental and applied aspects of molecular recognition and affinity technology in industry for health and disease, bioprocessing and biosensing.
Conferência
Affinity 2023 - 25th meeting of the International Society for Molecular Recognition, Portugal
2023/05/21 - 2023/05/28 The Laboratory of Crystallographic Studies (LEC) hosted the ISBC2023 in Granada (Spain) for the eighth time. The school provided five days of lectures and practical demonstrations on the crystallization of biological macromolecules, including large crystals for neutron diffraction and tiny crystals for XFEL or EM. One day was devoted to case studies on the crystallization of membrane proteins, viruses, large macromolecular complexes, and sample preparation for cryoEM.
Oficina (workshop)
8th International School of Biological Crystallization - ISBC2023, Spain
2022/09/25 - 2022/09/30 The main objective of the course is to provide the fundamental concepts and integrative hands-on applications of Macromolecular Crystallography (MX), Nuclear Magnetic Resonance (NMR), Magnetic Resonance Mass Spectrometry (MRMS) and single-particle analysis Cryo-Electron Microscopy (EM) in structural biology. The major strengths and limitations associated with each technique will be also highlighted and discussed along with their complementarity to achieve truly integrative structural biology approaches to tackle challenging science.
Oficina (workshop)
Joint Instruct-iNEXT Course: Integrating Structural Biology Techniques
Universidade Nova de Lisboa Instituto de Tecnologia Química e Biológica, Portugal
2020/08/09 - 2020/10/09 This annual meeting gathers synchrotron users, at a scientific event that includes presentations in plenary, poster, and parallel sessions. Participants are able to learn about current/future facility capabilities and the latest user research as well as to discuss science with colleagues from academia, research laboratories, and industry worldwide.
Conferência
2020 Virtual SSRL/LCLS Users' Meeting
Stanford Synchrotron Radiation Lightsource, Estados Unidos
2020/05/05 - 2020/06/02 This online course aims at young biochemists/biophysicists and researchers active in related structural methods with little or no experience in solution scattering. The course covered basics of SAXS, instrumentation, sample preparation, modelling techniques and complementary use with other methods.
Oficina (workshop)
Solution Scattering from Biological Macromolecules
European Molecular Biology Laboratory Hamburg, Alemanha
2020/02/20 - 2020/02/20 Practical and theoretical insights into ChemDraw and Reaxys software.
Oficina (workshop)
Chemistry Software Workshop: ChemDraw and Reaxys
Universidade Nova de Lisboa, Portugal
2019/11 - 2019/11 Protein purification workshop
Oficina (workshop)
Protein purification workshop: Technical tips & tricks Column replacement day
GE Healthcare Healthcare Systems, Países Baixos
2019/06 - 2019/06 “Rock the elements – The challenge” was an event where scientists, students and the general public were invited to produce a science communication video, aiming to disseminate scientific knowledge. 3rd place in “Rock The elements – the challange”
Universidade Nova de Lisboa, Portugal
2019/05/22 - 2019/05/24 International Structural Biology conference Instruct Biennial Structural Biology Conference: Deeper into the Cell
Instruct-ERIC, Espanha
2019/05/22 - 2019/05/22 Women in Science Workshop - Instruct-ERIC. Practical advice from leading women scientists in industry and academia and discussions about how Instruct-ERIC can support our community to progress in their careers.
Oficina (workshop)
Women in Science Workshop - Instruct-ERIC
INSTRUCT-ULTRA, Espanha
2019/03/22 - 2019/03/25 At the National Meeting of Chemistry Students (in Portuguese, Encontro Nacional de Estudantes de Química) all chemistry students can attend presentations of national and international speakers, as well as participate in workshops.
Conferência
VII National Chemistry Students Meeting (ENEQUI '19)
Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2019/03 - 2019/03 Leacture and laboratory activity to middle school students about the importance of the elements of the periodic table in our lives.
Exposição
Escola Básica e Secundária Professor Armando de Lucena, Portugal
2018/09/28 - 2018/09/28 The UCIBIO Annual Meeting counts with 3 keynote speakers, invited speakers from industry, Presentations and Flash Presentations on the Thematic Lines, and Poster Sessions.
Encontro
UCIBIO anual meeting 2018
Universidade Nova de Lisboa, Portugal
2018/03 - 2018/03 International science communication contest, sponsored by the British Council and integrated in the National Geographic Summit.
Exposição
Fame LAb
British Council Portugal, Portugal
2017/07/24 - 2017/07/25 Supramolecular Chemistry Workshop. 1) Basic principles of supramolecular chemistry and molecular recognition. 2) Practical Class: Determination of an association constant of an inclusion complex. 3) Molecular recognition of neutral and ionic molecules - case studies.
Oficina (workshop)
Supramolecular Chemistry Workshop
Universidade Nova de Lisboa, Portugal
2017/07 - 2017/07 Summer internship where students integrate a research team at IST and work in a project and learn about the workings of the institute, how to develop their ideas and do a presentation at the end.
Oficina (workshop)
Técnico Summer Internships 2017
Universidade de Lisboa Instituto Superior Técnico, Portugal

Expedição científica

Descrição da atividade Instituição / Organização
2022/09/05 - 2022/09/16 Human Norepinephrine Transporter expression trials were conducted in an insect cell system, under the supervision of Dr. Manuel Etzkorn, at the Institute of Physical Biology Heinrich-Heine-Universität and Dr. Aldino Viegas, at UCIBIO@NOVA. These tests were conducted under the scope of the project "Biochemical, functional and structural studies of the human norepinephrine transporter", 2021.09576.CBM. Heinrich-Heine-Universität Düsseldorf, Alemanha
2021/10/01 - 2021/12/01 Unravelling arsenite oxidation reaction mechanism through Time-resolved Crystallography. Experience in sample preparation and optimisation for serial crystallography; knowledge on serial data collection using fixed targets at the T-REXX endstation @ PETRA III, Hamburg; data processing and structure determination. Universität Hamburg Hamburg Centre for Ultrafast Imaging, Alemanha

Tutoria

Tópico Nome do aluno
2019/10 - 2019/10 Support in the supervision of a MSc Student in the Biomolecular Techniques subject Marlene Cunha
2019/10 - 2019/10 Support in the supervision of a MSc Student in the Biomolecular Techniques course Kleyde Delgado
2019/04 - 2019/04 Suport in the supervion of a BSc Student during a laboratory internship Rita Jesus
Distinções

Prémio

2023 Best Poster Award - ENURS 2023
9th National Meeting of Synchrotron Radiation Users - ENURS 2023, Portugal
2023 Best Poster Award - ISBC 2023
8th International School of Biological Crystallization, ISBC2023, Espanha
2023 Younger Investigator Award for Best Flash Presentation - Affinity 2023
• Affinity 2023 - 25th meeting of the International Society for Molecular Recognition, Portugal, Portugal
2020 EMBL Workshop fellowship
European Molecular Biology Laboratory, França
2020 Best Poster Award - In situ Structural Biology: From Cryo-EM to Integrative Modelling
European Molecular Biology Laboratory, Alemanha
2019 Instruct-ERIC Women in Science fellowship award
INSTRUCT-ULTRA, Espanha
2019 Best presentation at "Jornadas Intercalares dos mestrados do Departamento de Química e Ciências da Vida 2018/2019"
Universidade Nova de Lisboa Faculdade de Ciências e Tecnologia, Portugal
2019 3rd place in - Rock The elements - the challange

Título

2018 Semi-finalist at FameLAB contest
FameLab, Portugal

Outra distinção

2024 Travel Bursery
CCP4 Study Weekend 2024 , Reino Unido