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Anita Ferreira Triguinho da Silva is a passionate and dedicated biomedical scientist. She holds a bachelor's degree in Biomedical Sciences, with a final classification of 14 out of 20, awarded in June 2020 from the University of Algarve. She has been pursuing a master’s in Biomedical Sciences, at the same institution, finishing with the final classification of 17 out of 20. The final work of the degree, the monograph, was performed in the Stem Cell Biology group at the Centre for Biomedical Research of the University of Algarve. It was based on validating by qPCR the expression of some genes in response to impairing CITED2’s expression, in mouse embryonic stem cells (mESCs). During this project, she had the opportunity to learn how to maintain mESCs in cell culture, induce a conditional knockout and some lab techniques such as real-time RT-PCR. Her final grade was 17 out of 20. At the same laboratory, she did a volunteer period for approximately 20 months prior to developing her master’s thesis. During this time, she contributed as a co-first author, to a revised article about “Human Stem Cells for Cardiac Disease Modelling and Preclinical and Clinical Applications – Are We on the Road to Success?” (Ferreira et al., 2023, Cells), and was also a member of the “Non-invasive approach to limit congenital heart disease in mammalian embryos in utero” Mariano Gago by ABC project. Recently, she finished her master’s thesis project in the Chromosome Segregation in Disease Lab, at the Algarve Biomedical Center-Research Institute (ABC-Ri), obtaining a grade of 18 out of 20. During this research project, entitled “Study of BUB1 functions during neurodevelopment”, she learned many new lab techniques. The objective of this project was, to generate an induced pluripotent stem cell (iPSC) line from microcephalic patient cells and deeply analyse their neuronal differentiation process under monolayer conditions, into forebrain cells, comparing with the differentiation of healthy iPSCs. Anita has gained hands-on experience on reprogrammed cells using Sendai Virus with Yamanaka factors, which allowed her to generate two new cell strains, from microcephalic fibroblasts, which are already available to the community (Ferreira et al., 2024). In addition, she did iPSCs culture, 2D spontaneous differentiation, monolayer neuronal differentiation, qPCR, real-time RT-PCR, immunofluorescence, fluorescence microscopy and in vivo manipulation using chicken embryos. The last was used to study, how BUB1 inhibition by compound microinjection in primary brain vesicles, affects the neurodevelopment of the embryo. Her motivation has been recognised, so she has been selected at the competitive call for an MSc student fellowship at the BBBrain project and also, selected as an MSc student for an IEFP internship. Her work has contributed to the publication of two articles in distinct scientific journals, she has participated in scientific events, and scientific activities namely the institute's seminars and in-person presentations, as well as outreach activities and scientific workshops. At the Portuguese Genetic Human Society's 2023 Annual Meeting, her work was displayed as a poster (Jorge, Milagre, Ferreira et al. 2023). Outside of academic life, she worked as a lifeguard for some summer seasons, in the COVID-19 line by producing viral transporter media and as a volunteer for a study of “Avaliação de Microalbuminuria na População Portuguesa”.
Identificação

Identificação pessoal

Nome completo
Anita Ferreira Triguinho da Silva

Nomes de citação

  • Ferreira, Anita

Identificadores de autor

Ciência ID
8B15-9DE2-5346
ORCID iD
0009-0002-2285-988X

Endereços de correio eletrónico

  • anitaftsilva@gmail.com (Profissional)

Telefones

Telemóvel
  • 912771597 (Profissional)

Moradas

  • Rua das Pedreiras, nº4, Lapas-Torres Novas, 2350-105, Lapas- Torres Novas, Torres Novas , Portugal (Pessoal)

Domínios de atuação

  • Ciências Médicas e da Saúde - Ciências da Saúde - Ciências Biomédicas Sociais

Idiomas

Idioma Conversação Leitura Escrita Compreensão Peer-review
Português (Idioma materno)
Inglês Utilizador independente (B1) Utilizador independente (B2) Utilizador independente (B2) Utilizador independente (B2)
Formação
Grau Classificação
2020/09/01 - 2025/03
Concluído
Ciências Biomédicas - Mecanismos de doenças (Mestrado)
Universidade do Algarve Departamento de Ciências Biomédicas e Medicina, Portugal
"Study of BUB1 functions in the neurodevelopment" (TESE/DISSERTAÇÃO)
17
2016/09/01 - 2020/06/20
Concluído
Ciências Biomédicas (Licenciatura)
Universidade do Algarve Departamento de Ciências Biomédicas e Medicina, Portugal
"Validação por qPCR de genes diferencialmente expressos nas células estaminais embrionárias em resposta à diminuição da expressão de Cited2" (TESE/DISSERTAÇÃO)
14
Percurso profissional

Ciência

Categoria Profissional
Instituição de acolhimento
Empregador
2025/02 - Atual Investigador (Investigação) Algarve Biomedical Center Research Institute, Portugal
Algarve Biomedical Center Research Institute, Portugal
2023/04 - 2025/03 Estagiário de Investigação (Investigação) Algarve Biomedical Center Research Institute, Portugal
Algarve Biomedical Center Research Institute, Portugal
2024/04 - 2024/10 Investigador (Investigação) Algarve Biomedical Center Research Institute, Portugal
Algarve Biomedical Center Research Institute, Portugal
2021/09 - 2023/03 Estagiário de Investigação (Investigação) Algarve Biomedical Center Research Institute, Portugal
Algarve Biomedical Center Research Institute, Portugal
2020/11/01 - 2021/02/28 Assistente de Investigação (carreira) (Investigação) Centro Académico de Investigação e Formação Biomédica do Algarve, Portugal
Algarve Biomedical Center Research Institute, Portugal
Projetos

Outro

Designação Financiadores
2023/03 - Atual Study of BUB1 in neurodevelopment: identification of new functions
2022.01028.PTDC
Bolseiro de Investigação
Em curso
2023 - Atual NON-INVASIVE APPROACH TO LIMIT CONGENITAL HEART DISEASE IN MAMMALIAN EMBRYOS IN UTERO
Mariano Gago by ABC
Team Member
Em curso
Produções

Publicações

Artigo em revista
  1. Ferreira, Anita; Calado, Sofia M.; Jorge, Xavier; de Lange, Job; Carvalhal, Sara. "Generation and characterization of two isogenic induced pluripotent stem cell lines from a young female with microcephaly carrying a compound heterozygous mutation in BUB1 gene". Stem Cell Research 81 (2024): 103594. http://dx.doi.org/10.1016/j.scr.2024.103594.
    10.1016/j.scr.2024.103594
  2. Ferreira, Anita; Correia CD; Mónica T. Fernandes; Silva BM; Filipa Esteves; Leitão HS; José Bragança; Sofia Calado. "co-first author || Human Stem Cells for Cardiac Disease Modeling and Preclinical and Clinical Applications-Are We on the Road to Success?". Cells (2023): https://europepmc.org/articles/PMC10341347.
    10.3390/cells12131727
Poster em conferência
  1. Jorge, Xavier; Milagre, Inês; Ferreira, Anita; Calado, Sofia; Oliveira, Raquel; Carvalhal, Sara. "Common mechanistic pathways in rare congenital syndromes with primary microcephaly". Trabalho apresentado em 27th Annual Meeting - Sociedade Portuguesa de Genética Humana, 2023.
Atividades

Apresentação oral de trabalho

Título da apresentação Nome do evento
Anfitrião (Local do evento)
2024/10 INSTITUTIONAL SEMINAR AT ABC-RI: Explore BUB1 functions during neurodevelopment. Seminar open to the institutewhere MsC are invited to share their lastest updates. ABC-Ri Pizza Lab Club
2024/02 INSTITUTIONAL SEMINAR @ ABC-Ri: Explore BUB1 functions during neurodevelopment. Seminar open to the institute where MsC are invited to share their lastest updates. ABC-Ri Pizza Lab Club
2024/02 ORAL TALK at DevoStem Meeting @ ABC-Ri: BUB1 functions during neurodevelopment - new clues. ABC-Ri DevoStem

Orientação

Título / Tema
Papel desempenhado
Curso (Tipo)
Instituição / Organização
2024/03 - 2024/04 STUDENT GUIDANCE: BUB1 in chicken: tools to study BUB1 during neurodevelopment
Coorientador
Algarve Biomedical Center Research Institute, Portugal
2022 - 2022 STUDENT GUIDANCE - Diferenciação de células pluripotentes em células cardíacas - observational training under the Biomedical Science undergrad course (1 month).
Coorientador
Algarve Biomedical Center Research Institute, Portugal
2022 - 2022 STUDENT GUIDANCE - Knockout of the Cited2 protein in order to study the signaling pathways and feedback process involved with the p53 protein
Coorientador
Algarve Biomedical Center Research Institute, Portugal

Participação em evento

Descrição da atividade
Tipo de evento
Nome do evento
Instituição / Organização
2024/04 - Atual University of Algarve - OPEN DAY
Universidade do Algarve Departamento de Ciências Biomédicas e Medicina, Portugal
2022/11 - Atual A brief presentation on each of the center's research laboratories to the students of the Silves School.
Outro
Algarve Biomedical Center Research Institute, Portugal
2022/04 - Atual University of Algarve - OPEN DAY
Outro
Universidade do Algarve Departamento de Ciências Biomédicas e Medicina, Portugal
2021 - Atual University of Algarve - OPEN DAY
Outro
Universidade do Algarve Departamento de Ciências Biomédicas e Medicina, Portugal
2020/04 - Atual University of Algarve - OPEN DAY
Outro
Universidade do Algarve Departamento de Ciências Biomédicas e Medicina, Portugal
2024/04/11 - 2024/04/11 Hands-on workshop "Quantification in ImageJ"
Oficina (workshop)
Quantification in ImageJ
Algarve Biomedical Center Research Institute, Portugal
2024/03 - 2024/03 OUTREACH ACTIVITY during Brain Awarness Week -"Cérebro e Neurodiversidade"
2024/01/16 - 2024/01/16 46th BSCB GenSoc UK Cilia Network e-symposium
Seminário
46th BSCB GenSoc UK Cilia Network e-symposium
2023/10/17 - 2023/10/17 Abcam Cell Cycle conference
Seminário
Abcam Cell Cycle conference
Abcam plc, Reino Unido
2023 - 2023 OUTREACH ACTIVITY during Science and Tecnology week
Outro
Centro Ciência Viva do Algarve, Portugal
2022/10 - 2022/10 FIC.A- Festival Internacional de Ciência - Oeiras (International Science Festival )
Algarve Biomedical Center Research Institute, Portugal
2018/04 - 2018/04 Biomedical Sciences National CONFERENCE
Congresso
Universidade do Algarve Departamento de Ciências Biomédicas e Medicina, Portugal
2017/03 - 2017/03 Biomedical Sciences National CONFERENCE
Congresso
Universidade da Beira Interior, Portugal
Distinções

Outra distinção

2025 Selected at the competitive call for a MSc student IEFP' internship at the project BBBrain
2024 Selected at the competitive call for a MSc student fellowship at the project BBBrain