???global.info.a_carregar???
Miguel Mano graduated in Biochemistry from the University of Coimbra, Portugal, in 2001, and obtained his PhD degree in Biochemistry from the University of Coimbra, Portugal, in 2006. After the PhD, M. Mano worked as a Post-Doctoral fellow (2006-2008) at the Molecular Medicine laboratory and as Scientific Manager of the High-Throughput Screening Facility (2008-2014) at the International Centre for Genetic Engineering and Biotechnology (ICGEB, Trieste, Italy). In 2014, Miguel Mano established an independent laboratory at the Centre for Neuroscience and Cell Biology (CNC, Coimbra, Portugal), under the FCT Investigator Programme from the Portuguese Science Foundation (FCT, Portugal). Since 2021, M. Mano is Senior Lecturer and Head of High-Throughput/High-Content Screening at the British Heart Foundation Centre of Research Excellence and School of Cardiovascular Medicine & Sciences, King's College London, United Kingdom. His research is focused on two main areas: i) the identification and molecular characterization of novel cellular factors relevant to cardiac repair and regeneration, and the translation of this knowledge into effective RNA-based therapeutic strategies, and ii) the development of high-throughput and high-content screening technologies genome-wide siRNA, CRISPR and microRNA libraries. Miguel Mano has published over 50 papers in international peer-reviewed journals, including papers in high-impact factor journals (e.g. Nature, Nature Communications, Nature Cell Biology, Nature Microbiology, Cancer Cell, PNAS, EMBO Journal), which received over 3,800 citations; h-index 28. M. Mano is also a co-inventor of 2 patents related to the clinical development of miRNAs for cardiovascular diseases. M. Mano has obtained funding, as Principal Investigator, from different funding agencies (total funding exceeding 1.6M EUR since July 2014).
Ler resumo completo ↓
Identificação

Identificação pessoal

Nome completo
Miguel Luis Cunha Mano

Nomes de citação

  • Mano, Miguel

Identificadores de autor

Ciência ID
7A14-C491-2F79
ORCID iD
0000-0003-1922-4824
Scopus Author Id
8839364900

Moradas

  • CNC - Centro de Neurociências e Biologia Celular. UC-BIOTECH, Parque Tecnologico de Cantanhede, Núcleo 04, Lote 08, 3060-197, Cantanhede, Cantanhede, Portugal (Profissional)

Websites

Domínios de atuação

  • Ciências Médicas e da Saúde - Biotecnologia Médica - Diagnóstico e Terapias de Base Genética
  • Ciências Médicas e da Saúde - Biotecnologia Médica - Tecnologias que envolvem a Manipulação de Células,Tecidos,Órgãos ou todo o Organismo
  • Ciências Naturais - Ciências Biológicas - Biologia Molecular
  • Ciências Naturais - Ciências Biológicas - Biologia Celular
  • Ciências Médicas e da Saúde - Medicina Clínica - Sistemas Cardíacos e Cardiovasculares
Percurso profissional

Ciência

Categoria Profissional
Instituição de acolhimento 		Empregador
2018/03/01 - Atual Investigador principal (carreira) (Investigação) Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal
Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal
2014/07/01 - 2018/02/28 Investigador Auxiliar (carreira) (Investigação) Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal
Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal

Outros

Categoria Profissional
Instituição de acolhimento 		Empregador
2021/07/01 - Atual Senior Lecturer King's College London, Reino Unido
2021/07/01 - Atual Head, High-Throughput/High-Content Screening King's College London, Reino Unido
2011/11/01 - 2014/06/30 Staff Research Scientist International Centre for Genetic Engineering and Biotechnology, Itália
2009/01/01 - 2014/06/30 Scientific Manager, High-Throughput Screening Facility International Centre for Genetic Engineering and Biotechnology, Itália
2006/03/01 - 2008/12/31 Post Doctoral fellow International Centre for Genetic Engineering and Biotechnology, Itália
2001/09/01 - 2006/02/10 PhD student Universidade de Coimbra Centro de Neurociências e Biologia Celular, Portugal
Produções

Publicações

Artigo em revista
  1. Dinami, R.; Pompili, L.; Petti, E.; Porru, M.; D'Angelo, C.; Di Vito, S.; Rizzo, A.; et al. "MiR-182-3p targets TRF2 and impairs tumor growth of triple-negative breast cancer". EMBO Molecular Medicine 15 1 (2023):
    10.15252/emmm.202216033
  2. Ines Rodrigues Lopes; Laura Maria Alcantara; Ricardo Jorge Silva; Jerome Josse; Elena Pedrero Vega; Ana Marina Cabrerizo; Melanie Bonhomme; et al. "Microscopy-based phenotypic profiling of infection by Staphylococcus aureus clinical isolates reveals intracellular lifestyle as a prevalent feature". Nature Communications 13 1 (2022):
    10.1038/s41467-022-34790-9
  3. Carmen Aguilar; Susana Costa; Claire Maudet; R. P. Vivek-Ananth; Sara Zaldívar-López; Juan J. Garrido; Areejit Samal; Miguel Mano; Ana Eulalio. "Reprogramming of microRNA expression via E2F1 downregulation promotes Salmonella infection both in infected and bystander cells". Nature Communications (2021):
    10.1038/s41467-021-23593-z
  4. Clivia Lisowski; Jane Dias; Susana Costa; Ricardo Jorge Silva; Miguel Mano; Ana Eulalio. "Dysregulated endolysosomal trafficking in cells arrested in the G1 phase of the host cell cycle impairs Salmonella vacuolar replication". Autophagy (2021):
    10.1080/15548627.2021.1999561
  5. João Paulo Silva Nunes; Pauline Andrieux; Pauline Brochet; Rafael Ribeiro Almeida; Eduardo Kitano; André Kenji Honda; Leo Kei Iwai; et al. "Co-Exposure of Cardiomyocytes to IFN-¿ and TNF-a Induces Mitochondrial Dysfunction and Nitro-Oxidative Stress". Frontiers in Immunology 12 (2021):
    10.3389/fimmu.2021.755862
  6. Aguilar, C.; Cruz, A.R.; Rodrigues Lopes, I.; Maudet, C.; Sunkavalli, U.; Silva, R.J.; Sharan, M.; et al. "Functional screenings reveal different requirements for host microRNAs in Salmonella and Shigella infection". Nature Microbiology 5 1 (2020): 192-205. # co-last author.
    10.1038/s41564-019-0614-3
  7. Sorino, C.; Catena, V.; Bruno, T.; De Nicola, F.; Scalera, S.; Bossi, G.; Fabretti, F.; et al. "Che-1/AATF binds to RNA polymerase I machinery and sustains ribosomal RNA gene transcription". Nucleic Acids Research 48 11 (2020): 5891-5906.
    10.1093/NAR/GKAA344
  8. Bertolio, R.; Napoletano, F.; Mano, M.; Maurer-Stroh, S.; Fantuz, M.; Zannini, A.; Bicciato, S.; Sorrentino, G.; Del Sal, G.. "Sterol regulatory element binding protein 1 couples mechanical cues and lipid metabolism". Nature Communications 10 1 (2019):
    10.1038/s41467-019-09152-7
  9. Aguilar, C.; Mano, M.; Eulalio, A.. "Multifaceted roles of microRNAs in host-bacterial pathogen interaction". Microbiology Spectrum 7 3 (2019):
    10.1128/microbiolspec.BAI-0002-2019
  10. De Souza Lima, I.M.; Schiavinato, J.L.D.S.; Paulino Leite, S.B.; Sastre, D.; Bezerra, H.L.D.O.; Sangiorgi, B.; Corveloni, A.C.; et al. "High-content screen in human pluripotent cells identifies miRNA-regulated pathways controlling pluripotency and differentiation". Stem Cell Research and Therapy 10 1 (2019):
    10.1186/s13287-019-1318-6
  11. Ali, H.; Mano, M.; Braga, L.; Naseem, A.; Marini, B.; Vu, D.M.; Collesi, C.; et al. "Cellular TRIM33 restrains HIV-1 infection by targeting viral integrase for proteasomal degradation". Nature Communications 10 1 (2019):
    10.1038/s41467-019-08810-0
  12. Torrini, C.; Cubero, R.J.; Dirkx, E.; Braga, L.; Ali, H.; Prosdocimo, G.; Gutierrez, M.I.; et al. "Common Regulatory Pathways Mediate Activity of MicroRNAs Inducing Cardiomyocyte Proliferation". Cell Reports 27 9 (2019): 2759-2771.e5.
    10.1016/j.celrep.2019.05.005
  13. Rodrigues Lopes, I.; Silva, R.J.; Caramelo, I.; Eulalio, A.; Mano, M.. "Shedding light on microRNA function via microscopy-based screening". Methods 152 (2019): 55-64.
    10.1016/j.ymeth.2018.09.011
  14. Aguilar, C.; Mano, M.; Eulalio, A.. "MicroRNAs at the Host–Bacteria Interface: Host Defense or Bacterial Offense". Trends in Microbiology 27 3 (2019): 206-218.
    10.1016/j.tim.2018.10.011
  15. Bellazzo, A.; Di Minin, G.; Valentino, E.; Sicari, D.; Torre, D.; Marchionni, L.; Serpi, F.; et al. "Cell-autonomous and cell non-autonomous downregulation of tumor suppressor DAB2IP by microRNA-149-3p promotes aggressiveness of cancer cells". Cell Death and Differentiation 25 7 (2018): 1224-1238.
    10.1038/s41418-018-0088-5
  16. Andriani, F.; Majorini, M.T.; Mano, M.; Landoni, E.; Miceli, R.; Facchinetti, F.; Mensah, M.; et al. "MiR-16 regulates the pro-tumorigenic potential of lung fibroblasts through the inhibition of HGF production in an FGFR-1- and MEK1-dependent manner". Journal of Hematology and Oncology 11 1 (2018):
    10.1186/s13045-018-0594-4
  17. Majorini, M.T.; Manenti, G.; Mano, M.; De Cecco, L.; Conti, A.; Pinciroli, P.; Fontanella, E.; et al. "CIAP1 regulates the EGFR/Snai2 axis in triple-negative breast cancer cells". Cell Death and Differentiation 25 12 (2018): 2147-2164.
    10.1038/s41418-018-0100-0
  18. Tawk, C.; Nigro, G.; Rodrigues Lopes, I.; Aguilar, C.; Lisowski, C.; Mano, M.; Sansonetti, P.; Vogel, J.; Eulalio, A.. "Stress-induced host membrane remodeling protects from infection by non-motile bacterial pathogens". EMBO Journal 37 23 (2018):
    10.15252/embj.201798529
  19. Ingallina, E.; Sorrentino, G.; Bertolio, R.; Lisek, K.; Zannini, A.; Azzolin, L.; Severino, L.U.; et al. "Mechanical cues control mutant p53 stability through a mevalonate-RhoA axis". Nature Cell Biology 20 1 (2018): 28-35.
    10.1038/s41556-017-0009-8
  20. Cunningham, T.J.; Yu, M.S.; McKeithan, W.L.; Spiering, S.; Carrette, F.; Huang, C.-T.; Bushway, P.J.; et al. "Id genes are essential for early heart formation". Genes and Development 31 13 (2017): 1325-1338.
    10.1101/gad.300400.117
  21. Sunkavalli, U.; Aguilar, C.; Silva, R.J.; Sharan, M.; Cruz, A.R.; Tawk, C.; Maudet, C.; Mano, M.; Eulalio, A.. "Analysis of host microRNA function uncovers a role for miR-29b-2-5p in Shigella capture by filopodia". PLoS Pathogens 13 4 (2017):
    10.1371/journal.ppat.1006327
  22. Sorrentino, G.; Ruggeri, N.; Zannini, A.; Ingallina, E.; Bertolio, R.; Marotta, C.; Neri, C.; et al. "Glucocorticoid receptor signalling activates YAP in breast cancer". Nature Communications 8 (2017):
    10.1038/ncomms14073
  23. Dinami, R.; Buemi, V.; Sestito, R.; Zappone, A.; Ciani, Y.; Mano, M.; Petti, E.; et al. "Epigenetic silencing of miR-296 and miR-512 ensures hTERT dependent apoptosis protection and telomere maintenance in basal-type breast cancer cells". Oncotarget 8 56 (2017): 95674-95691.
    10.18632/oncotarget.21180
  24. Cunha, P.P.; Costa, P.M.; Morais, C.M.; Lopes, I.R.; Cardoso, A.M.; Cardoso, A.L.; Mano, M.; Jurado, A.S.; Pedroso De Lima, M.C.P.. "High-throughput screening uncovers miRNAs enhancing glioblastoma cell susceptibility to tyrosine kinase inhibitors". Human Molecular Genetics 26 22 (2017): 4375-4387.
    10.1093/hmg/ddx323
  25. Conti, A.; Majorini, M.T.; Fontanella, E.; Bardelli, A.; Giacca, M.; Delia, D.; Mano, M.; Lecis, D.. "Lemur tyrosine kinase 2 (LMTK2) is a determinant of cell sensitivity to apoptosis by regulating the levels of the BCL2 family members". Cancer Letters 389 (2017): 59-69.
    10.1016/j.canlet.2016.12.025
  26. Eulalio, A.; Mano, M.. "MicroRNA Screening and the Quest for Biologically Relevant Targets". Journal of Biomolecular Screening 20 8 (2015): 1003-1017.
    10.1177/1087057115578837
  27. Mano, M.; Ippodrino, R.; Zentilin, L.; Zacchigna, S.; Giacca, M.. "Genome-wide RNAi screening identifies host restriction factors critical for in vivo AAV transduction". Proceedings of the National Academy of Sciences of the United States of America 112 36 (2015): 11276-11281. # corresponding author.
    10.1073/pnas.1503607112
  28. Costa, P.M.; Cardoso, A.L.; Mano, M.; de Lima, M.C.P.. "MicroRNAs in glioblastoma: Role in pathogenesis and opportunities for targeted therapies". CNS and Neurological Disorders - Drug Targets 14 2 (2015): 222-238.
    10.2174/1871527314666150116123610
  29. Peche, L.Y.; Ladelfa, M.F.; Toledo, M.F.; Mano, M.; Laiseca, J.E.; Schneider, C.; Monte, M.. "Human MageB2 protein expression enhances E2F transcriptional activity, cell proliferation, and resistance to ribotoxic stress". Journal of Biological Chemistry 290 49 (2015): 29652-29662.
    10.1074/jbc.M115.671982
  30. Maudet, C.; Mano, M.; Eulalio, A.. "MicroRNAs in the interaction between host and bacterial pathogens". FEBS Letters 588 22 (2014): 4140-4147.
    10.1016/j.febslet.2014.08.002
  31. Sorrentino, G.; Ruggeri, N.; Specchia, V.; Cordenonsi, M.; Mano, M.; Dupont, S.; Manfrin, A.; et al. "Metabolic control of YAP and TAZ by the mevalonate pathway". Nature Cell Biology 16 4 (2014): 357-366.
    10.1038/ncb2936
  32. Arnoldi, F.; De Lorenzo, G.; Mano, M.; Schraner, E.M.; Wild, P.; Eichwald, C.; Burrone, O.R.. "Rotavirus increases levels of lipidated LC3 supporting accumulation of infectious progeny virus without inducing autophagosome formation". PLoS ONE 9 4 (2014):
    10.1371/journal.pone.0095197
  33. Zecchin, A.; Pattarini, L.; Gutierrez, M.I.; Mano, M.; Mai, A.; Valente, S.; Myers, M.P.; Pantano, S.; Giacca, M.. "Reversible acetylation regulates vascular endothelial growth factor receptor-2 activity". Journal of Molecular Cell Biology 6 2 (2014): 116-127.
    10.1093/jmcb/mju010
  34. Verardo, R.; Piazza, S.; Klaric, E.; Ciani, Y.; Bussadori, G.; Marzinotto, S.; Mariuzzi, L.; et al. "Specific mesothelial signature marks the heterogeneity of mesenchymal stem cells from high-grade serous ovarian cancer". Stem Cells 32 11 (2014): 2998-3011.
    10.1002/stem.1791
  35. Maudet, C.; Mano, M.; Sunkavalli, U.; Sharan, M.; Giacca, M.; Förstner, K.U.; Eulalio, A.. "Functional high-throughput screening identifies the miR-15 microRNA family as cellular restriction factors for Salmonella infection". Nature Communications 5 (2014):
    10.1038/ncomms5718
  36. Ottaviani, G.; Gobbo, M.; Sturnega, M.; Martinelli, V.; Mano, M.; Zanconati, F.; Bussani, R.; et al. "Effect of class IV laser therapy on chemotherapy-induced oral mucositis: A clinical and experimental study". American Journal of Pathology 183 6 (2013): 1747-1757.
    10.1016/j.ajpath.2013.09.003
  37. Carrer, A.; Moimas, S.; Zacchigna, S.; Pattarini, L.; Zentilin, L.; Ruozi, G.; Mano, M.; et al. "Neuropilin-1 identifies a subset of bone marrow Gr1-monocytes that can induce tumor vessel normalization and inhibit tumor growth". Cancer Research 72 24 (2012): 6371-6381.
    10.1158/0008-5472.CAN-12-0762
  38. Eulalio, A.; Mano, M.; Ferro, M.D.; Zentilin, L.; Sinagra, G.; Zacchigna, S.; Giacca, M.. "Functional screening identifies miRNAs inducing cardiac regeneration". Nature 492 7429 (2012): 376-381.
    10.1038/nature11739
  39. Lovric, J.; Mano, M.; Zentilin, L.; Eulalio, A.; Zacchigna, S.; Giacca, M.. "Terminal differentiation of cardiac and skeletal myocytes induces permissivity to AAV transduction by relieving inhibition imposed by DNA damage response proteins". Molecular Therapy 20 11 (2012): 2087-2097.
    10.1038/mt.2012.144
  40. de Lorenzo, G.; Eichwald, C.; Schraner, E.M.; Nicolin, V.; Bortul, R.; Mano, M.; Burrone, O.R.; Arnoldi, F.. "Production of in vivo-biotinylated rotavirus particles". Journal of General Virology 93 PART 7 (2012): 1474-1482.
    10.1099/vir.0.040089-0
  41. Contin, R.; Arnoldi, F.; Mano, M.; Burrone, O.R.. "Rotavirus replication requires a functional proteasome for effective assembly of viroplasms". Journal of Virology 85 6 (2011): 2781-2792.
    10.1128/JVI.01631-10
  42. Girardini, J.; Napoli, M.; Piazza, S.; Rustighi, A.; Marotta, C.; Radaelli, E.; Capaci, V.; et al. "A Pin1/Mutant p53 Axis Promotes Aggressiveness in Breast Cancer". Cancer Cell 20 1 (2011): 79-91.
    10.1016/j.ccr.2011.06.004
  43. Eulalio, A.; Fröhlich, K.S.; Mano, M.; Giacca, M.; Vogel, J.. "A candidate approach implicates the secreted Salmonella effector protein SpvB in P-body disassembly". PLoS ONE 6 3 (2011):
    10.1371/journal.pone.0017296
  44. Murgia, S.; Falchi, A.M.; Mano, M.; Lampis, S.; Angius, R.; Carnerup, A.M.; Schmidt, J.; et al. "Nanoparticles from lipid-based liquid crystals: Emulsifier influence on morphology and cytotoxicity". Journal of Physical Chemistry B 114 10 (2010): 3518-3525.
    10.1021/jp9098655
  45. Trabulo, S.; Cardoso, A.L.; Mano, M.; de Lima, M.C.P.. "Cell-penetrating peptides-mechanisms of cellular uptake and generation of delivery systems". Pharmaceuticals 3 4 (2010): 961-993.
    10.3390/ph3040961
  46. Padari, K.; Koppel, K.; Lorents, A.; Hällbrink, M.; Mano, M.; Pedroso De Lima, M.C.; Pooga, M.. "S413-PV cell-penetrating peptide forms nanoparticle-like structures to gain entry into cells". Bioconjugate Chemistry 21 4 (2010): 774-783.
    10.1021/bc900577e
  47. Trabulo, S.; Mano, M.; Faneca, H.; Cardoso, A.L.; Duarte, S.; Henriques, A.; Paiva, A.; et al. "S413-PV cell penetrating peptide and cationic liposomes act synergistically to mediate intracellular delivery of plasmid DNA". Journal of Gene Medicine 10 11 (2008): 1210-1222.
    10.1002/jgm.1247
  48. Cervelli, T.; Palacios, J.A.; Zentilin, L.; Mano, M.; Schwartz, R.A.; Weitzman, M.D.; Giacca, M.. "Processing of recombinant AAV genomes occurs in specific nuclear structures that overlap with foci of DNA-damage-response proteins". Journal of Cell Science 121 3 (2008): 349-357.
    10.1242/jcs.003632
  49. Mano, M.; Henriques, A.; Paiva, A.; Prieto, M.; Gavilanes, F.; Simões, S.; Pedroso de Lima, M.C.. "Interaction of S413-PV cell penetrating peptide with model membranes: Relevance to peptide translocation across biological membranes". Journal of Peptide Science 13 5 (2007): 301-313.
    10.1002/psc.842
  50. Mano, M.; Henriques, A.; Paiva, A.; Prieto, M.; Gavilanes, F.; Simões, S.; Pedroso de Lima, M.C.. "Cellular uptake of S413-PV peptide occurs upon conformational changes induced by peptide-membrane interactions". Biochimica et Biophysica Acta - Biomembranes 1758 3 (2006): 336-346.
    10.1016/j.bbamem.2006.01.014
  51. Mano, M.; Teodósio, C.; Paiva, A.; Simões, S.; Pedroso De Lima, M.C.. "On the mechanisms of the internalization of S413-PV cell-penetrating peptide". Biochemical Journal 390 2 (2005): 603-612.
    10.1042/BJ20050577
  52. Simões, S.; Filipe, A.; Faneca, H.; Mano, M.; Penacho, N.; Düzgünes, N.; de Lima, M.P.. "Cationic liposomes for gene delivery". Expert Opinion on Drug Delivery 2 2 (2005): 237-254.
    10.1517/17425247.2.2.237
  53. Piedade, J.A.P.; Mano, M.; Pedroso de Lima, M.C.; Oretskaya, T.S.; Oliveira-Brett, A.M.. "Voltammetric behaviour of oligonucleotide lipoplexes adsorbed onto glassy carbon electrodes". Journal of Electroanalytical Chemistry 564 1-2 (2004): 25-34.
    10.1016/j.jelechem.2003.10.008
  54. Piedade, J.A.P.; Mano, M.; Pedroso De Lima, M.C.; Oretskaya, T.S.; Oliveira-Brett, A.M.. "Electrochemical sensing of the behaviour of oligonucleotide lipoplexes at charged interfaces". Biosensors and Bioelectronics 20 5 (2004): 975-984.
    10.1016/j.bios.2004.06.014
Capítulo de livro
  1. Carmen Aguilar; Miguel Mano; Ana Eulalio. "Multifaceted roles of MicroRNAs in host-bacterial pathogen interaction". 247-266. WILEY-BLACKWELL, 2020.
    10.1128/9781683670261.ch17
  2. Pedroso De Lima, M.C.; Faneca, H.; Mano, M.; Penacho, N.; Düzgünes, N.; Simões, S.. "Biophysical characterization of cationic liposome-DNA complexes and their interaction with cells". 2003.
    10.1016/S0076-6879(03)73019-5
Tese / Dissertação
  1. Mano, Miguel. "Cell Penetrating Peptides as Promising Systems for Nucleic Acid Delivery: Mechanisms of Cellular Uptake and Biological Activity of S4(13)-PV Peptide". Doutoramento, Universidade de Coimbra Centro de Neurociências e Biologia Celular, 2006.
  2. Mano, Miguel. "Evaluation of the role of transferrin in overcoming the biological barriers faced by lipoplexes". Licenciatura, Universidade de Coimbra Centro de Neurociências e Biologia Celular, 2001.

Propriedade Intelectual

Patente
  1. Eulalio, Ana; Mano, Miguel; Zacchigna, Serena; Giacca, Mauro. 2017. "MicroRNAs for cardiac regeneration through induction of cardiac myocyte proliferation".
    Concedida/Emitida
  2. Braga, Luca; Dal Ferro, Matteo; Mano, Miguel; Eulalio, Ana; Giacca, Mauro. 2017. "MicroRNA hsa-miR-665 in cardiac hypertrophy".